Cell· Donghui Li, Alberto De Iaco, Kevin C. Ngan, Georgy Smyshlyaev, Ninad Kumbhojkar, Barrett E. Steinberg, Zohra Kalani, Lingbo Xia, Alexas Dolbee, Emily S. Kibbler, Miles C. Duncan, Ian Lin, Matthew S. Benczkowski, Lavanya Venkatesan, Andrea Calabria, Thomas A. Carter, Giulia I. Corsi, Trine A. Kristiansen, Gregory McAllister, Christopher Chase Bolt, Kimberly A. Harris, Jeffrey I. Boucher, Chunxi Zeng, Isha S. Dakhara, Xijia Wang, Shanmugapriya Sothiselvam, Ersin Akinci, Justin Strickland, Anubhuti Mathur, Christina Ferren, Jessica Desmond, Jason Rodriguez, James B. Rottman, Mohit Gupta, Lei Liu, Rahul Palchaudhuri, Z. Jane Wang, Robert J. Citorik, Jacob R. Rubens, Narahari S. Pujar, Michael C. Holmes, Cecilia Cotta-Ramusino·· 17 小时前精选AI 评分72
Cell：工程化逆转座子实现体内基因写入
In vivo Gene Writing with engineered retrotransposons
AI 导读
研究通过计算重建、筛选和工程化改造非 LTR 逆转座子，获得称为 Gene Writers 的元件，可在人细胞中借助全 RNA 递送实现稳定的转基因整合。经过表征与优化后，Gene Writers 在人源化小鼠模型中于体内生成了功能性 CAR-T 细胞。
阅读原文
来源：Cell · cell.com
推荐理由
该工作通过计算重建与筛选改造非 LTR 逆转座子，为全 RNA 递送的稳定转基因整合提供了新的候选体系。