RNAffinityDB 发布实验测定的 RNA-蛋白结合亲和力数据库
RNAffinityDB: a curated database of experimental RNA–protein binding affinities
RNAffinityDB 是一个人工整理的实验 RNA-蛋白结合亲和力数据库，每条测量都锚定到一个明确的蛋白构建体和一个由规范核糖核苷酸组成的单链 RNA 序列。当前版本收录来自 1,055 篇原始文献的 10,016 条构建体级记录，覆盖 3,057 个蛋白构建体和 4,638 条 RNA 序列。数据库按原文保留亲和力数值与测量边界，并收录未结合的观测结果。网页界面支持按蛋白身份或 RNA 序列直接检索，也支持按生物学功能进行语义查询，记录链接到 UniProt 注释，并提供 AlphaFold 3 预测复合物结构的交互式可视化。
来源：Nucleic Acids Research · doi.org
RNA–protein interactions modulate RNA function and direct RNA fate in cells. Over the past several decades, experimental studies have yielded a substantial but fragmented record of binding affinity measurements for defined RNA–protein pairs, obscuring a unified view of how sequence determinants shape binding strength. Here, we present RNAffinityDB, a curated database of experimental RNA–protein binding affinities. Its curation framework anchors each measurement to one defined protein construct and one single-stranded RNA with a defined sequence of canonical ribonucleotides. The current release comprises 10 ,016 construct-resolved records from 1,055 source publications, covering 3,057 protein constructs and 4,638 RNA sequences. The database preserves affinity values and measurement boundaries as reported, together with non-binding observations. The web interface enables direct retrieval by protein identity or RNA sequence and semantic queries on biological function, with records linked to UniProt annotations and interactive visualizations of AlphaFold 3-predicted complex structures. RNAffinityDB supports systematic analysis of sequence-affinity relationships and the downstream development of RNA–protein affinity models. RNAffinityDB is freely available at https://www.ultrarnalab.com/RNAffinityDB.php.