G4All 数据库发布经实验验证的 G-四链体形成序列
G4All: a database of experimentally confirmed G-quadruplex-forming sequences
G4All 是一个经人工整理、收录实验验证的 G-四链体（G4）形成短 DNA 与 RNA 序列的数据库，并配有严格筛选的非 G4 对照序列，这些序列大多在约 100 mM 钾离子、接近中性 pH 的相近实验条件下研究。数据库整合了圆二色谱、NMR、紫外光谱等多种实验方法验证的序列，并标注拓扑结构、热稳定性和实验条件。正负数据集并存，使研究者可进行严格的比较分析、实验方法基准评测和预测模型开发，应用覆盖 G4 生物物理基础研究、适配体理性设计以及预测算法基准测试。
来源：Nucleic Acids Research · doi.org
G-quadruplexes (G4s) are non-canonical nucleic acid structures with critical roles in gene regulation, genomic stability, and disease, making them prime targets for therapeutic and biotechnological applications. However, the absence of a curated, centralized, experimentally validated repository of short (mostly synthetic) G4-forming sequences, paired with appropriate single-stranded or hairpin controls, has limited reproducibility and hindered progress in the field. Here, we introduce G4All, a comprehensive, curated database of G4-forming short DNA and RNA sequences, complemented by rigorously selected non-G4 controls, all studied under roughly similar experimental conditions (generally around 100 mM potassium and near neutral pH). G4All consolidates sequences validated by diverse experimental methods, including circular dichroism, NMR, UV spectroscopy, with annotations for topology, thermal stability, and experimental conditions. By providing both positive and negative datasets, G4All enables rigorous comparative analyses, assay benchmarking, and the development of predictive models. The database supports a broad range of applications, from fundamental studies of G4 biophysics to the rational design of aptamers, as well as benchmarking prediction algorithms. Future developments will expand G4All to include user-submitted datasets and more RNA sequences. Freely accessible at https://g4-all.streamlit.app/, G4All offers a searchable interface and downloadable datasets, establishing a community-driven hub to accelerate discovery and standardization in G4 research.