PrEditR 发布：面向蛋白残基的 CRISPR 碱基编辑 sgRNA 设计平台
PrEditR: A protein-centric platform for CRISPR-mediated base editor sgRNA design
PrEditR 是一个开源、以蛋白为中心的高通量 sgRNA 设计工具，用于定制碱基编辑筛选。用户可指定蛋白中的特定氨基酸残基，工具据此设计靶向对应基因的 protospacer 序列，并注释每个产生的氨基酸变化（错义、无义或同义），包括 bystander 编辑。
来源：Bioinformatics Advances · doi.org
Motivation
Post-translational modifications (PTMs) are critical to protein activity, yet the biological role of most known modification sites remains uncharacterized. CRISPR-mediated phenotypic screens using base editors offer a powerful approach to dissecting PTM function at scale. However, existing sgRNA design tools for base editing applications are DNA-centric and lack the throughput required to integrate seamlessly with mass-spectrometry-based proteomics experimental outputs.
Results
We introduce protein editing in R, PrEditR, an open-source, protein-centric tool for high-throughput sgRNA design for custom base editor screens. PrEditR enables users to designate specific amino acid residues in proteins and design protospacer sequences that target the corresponding gene and annotate every resulting amino acid change (missense, nonsense, or silent), including bystander edits.
Availability and Implementation
PrEditR is available on GitHub and Docker Hub.