WasteFams 发布全球污水微生物组蛋白家族数据库
WasteFams: a database of protein families from global wastewater microbiomes
WasteFams 是一个系统收录全球污水宏基因组与宏转录组蛋白家族的数据库。研究整合了 580 个宏基因组、132 个宏转录组和 1709 个参考基因组，过滤后保留 504 个宏基因组、129 个宏转录组和 1151 个参考基因组，从超过 1.05 亿条预测蛋白中整理出 3887 个非冗余蛋白家族（每个家族至少含 100 个成员）。每个家族附带 AlphaFold3 结构预测、分类学归属和生物群系元数据，并通过深度基因组上下文分析关联移动遗传元件、生物合成基因簇、抗生素抗性基因和 CRISPR 元件。
来源：NAR Genomics and Bioinformatics · doi.org
Wastewater surveillance has emerged as a critical tool for global epidemiology, yet the functional diversity of wastewater microbiomes remains poorly characterized at the protein level. Here, we present WasteFams, the first comprehensive database dedicated to the systematic exploration of protein families in wastewater metagenomic and metatranscriptomic studies worldwide. Integrating data from 580 metagenomes, 132 metatranscriptomes, and 1709 reference genomes (504 metagenomes, 129 metatranscriptomes, and 1151 reference genomes after filtering), WasteFams catalogs 3887 non-redundant protein families (containing ⪰100 members) derived from over 105 million predicted proteins. Each protein family is enriched with multi-layered annotations, including AlphaFold3 structural predictions, taxonomic classifications, and biome-specific metadata. To further expand their functional annotation, we integrated deep genomic context analysis to link protein families to mobile genetic elements, biosynthetic gene clusters, antibiotic resistance genes, and CRISPR elements. Accessible through the EnvoFams portal, WasteFams provides a user-friendly interface featuring advanced search capabilities, sequence and structural similarity tools, and interactive visualization modules. As global initiatives increasingly leverage wastewater for public health and environmental insights, WasteFams can serve as a critical resource for discovering novel microbial functions, monitoring resistance mechanisms, and exploring the biotechnological potential of secondary metabolites within wastewater-engineered ecosystems.