scTHREAD 发布细胞分辨长读长转录组数据库，整合 92 万余细胞
scTHREAD: a cell-resolved long-read transcriptome database with linked isoform, poly(A), junction, and allelic evidence
scTHREAD 是一个开放获取的单细胞长读长转录组数据库，用于整合、探索和检索单细胞长读长转录证据。它收录 34 个人与小鼠数据集的 453 个 run，覆盖 923 389 个由 Oxford Nanopore 和 PacBio 测序的细胞；统一且平台感知的流程编目了 12 471 531 条 run–转录本观测，其中 30% 带有 210 341 个不同 Ensembl 转录本标识符之一。异构体与 poly(A) 位点使用、剪接连接支持和等位基因感知计数均与来源研究、run、细胞类型和单细胞保持关联，覆盖度允许时还预计算了差异使用。
来源：Nucleic Acids Research · doi.org
该数据库把分散的单细胞长读长数据统一到同一流程下，并保留每条记录到细胞与研究的溯源，便于比较不同平台与队列的异构体使用。
Cell-resolved transcript structures are essential for understanding how isoform usage, alternative polyadenylation, and allelic expression vary across cell types. However, existing single-cell long-read datasets remain fragmented across studies, platforms, species, and workflows, hindering comparison and reuse. Here, we present scTHREAD (single-cell Transcriptome Hub for Long-Read Evidence, Annotation, and Discovery; https://scthread.ai4sc.ac.cn/), an open-access database for integrating, exploring, and retrieving single-cell long-read transcriptomic evidence. It comprises 453 runs from 34 human and mouse datasets, covering 923 389 cells profiled by Oxford Nanopore and PacBio. A uniform, platform-aware workflow catalogues 12 471 531 run–transcript observations, of which 30% carry one of 210 341 distinct Ensembl transcript identifiers. Isoform and poly(A)-site usage, splice-junction support, and allele-aware counts remain linked to their source study, run, cell type, and single cell, ensuring full provenance, and differential usage is precomputed where coverage permits. Records are explored through a web interface and retrieved through bulk downloads or a documented API. scTHREAD recapitulates known cell-type-specific isoform patterns and identifies reproducible monocyte–T-cell differences in CCND3, CUTA, and PSMB8 junction usage across three cohorts and two sequencing technologies, establishing it as a traceable resource for investigating RNA processing and transcript regulation at isoform resolution.