FITdb 发布整合功能免疫基因组学与转录组学数据库
FITdb, an integrated Functional Immunogenomics and Transcriptomics database
研究团队开发了可免费访问的功能免疫基因组学与转录组学数据库 FITdb，用于整合免疫细胞功能基因组学数据。FITdb 目前收录 43 项独立功能遗传筛选，其中 31 项为 bulk 筛选、12 项为单细胞筛选，覆盖 20 696 个小鼠基因和 22 293 个人类基因，涉及 199 种免疫细胞类型与细胞状态、29 种实验条件。所有数据集都用统一流程重新分析，以支持跨研究探索；数据库提供以基因为中心的可视化、单项筛选的详细浏览以及 single-guide RNA 层级数据。
来源：Nucleic Acids Research · doi.org
Genetic screens in immune cells enable the systematic interrogation of gene function at scale, uncovering key regulators of cell functions such as tumor cell killing and persistence. However, existing datasets typically focus on specific biological questions, employ targeted gene panels, are generated under diverse experimental conditions, and are not readily accessible, which together limit their integration and future usability. To address this, we developed the Functional Immunogenomics and Transcriptomics database (FITdb), a freely accessible resource that harmonizes functional genomics datasets for the study of immune cell biology. FITdb currently integrates 43 independent functional genetics screens, including 31 bulk and 12 single-cell screens, spanning 20 696 mouse genes and 22 293 human genes across 199 immune cell types and cell states across 29 conditions. Datasets are reanalyzed using a standardized workflow to facilitate cross-study exploration. FITdb provides intuitive, gene-centric visualizations, detailed exploration of individual screens, and access to single-guide RNA-level data. Additionally, built-in tools such as “Compare MyGeneSet” and “Compare MyScreen” identify statistically significant overlaps between user-defined gene lists and functional gene sets in FITdb, and enable direct comparison of user-generated screening data with existing datasets, respectively. Together, FITdb provides a curated, continuously expanding, and user-friendly platform for accelerating the discovery of immune regulatory programs. The database is freely available at https://fitdb.lji.org.