CAnDi 发布全球厌氧消化微生物组目录
CAnDi: a global catalogue of anaerobic digestion microbiomes
研究团队发布厌氧消化微生物目录 CAnDi，从全球 1256 份反应器宏基因组样本中重建出 36 480 个原核基因组，覆盖 11 149 个物种。这些宏基因组还编码约 190 万条病毒序列和超过 13 万条质粒序列；聚类分析得到约 53.6 万个候选病毒物种，其中 85% 相对现有数据库为新，并识别出超过 15 000 个独特质粒。作者从头构建了病毒与质粒蛋白家族聚类，分别得到 1 721 182 和 123 813 个不同蛋白家族。作者称该资源扩展了此前对厌氧消化微生物组的系统刻画，为研究产甲烷生态系统中病毒组和质粒组的生态与演化作用提供基础。
来源：Nucleic Acids Research · doi.org
Methanogenic environments harbour complex prokaryotic consortia that drive the anaerobic conversion of organic matter into methane and carbon dioxide. Beyond being key to the global carbon cycle, such processes have been steered into powerful biotechnologies for energy production in bioreactors. Decoding the functional and taxonomic landscape of these communities requires comprehensive surveys, including bacteriophages and plasmids, which, however, remain vastly underrepresented in current databases. Here, we present the microbial Catalogue of Anaerobic Digestion (CAnDi), a database comprising 36 480 prokaryotic genomes spanning 11 149 species recovered from 1256 metagenomic samples collected across bioreactors worldwide. The reconstructed metagenomes also encode ∼1.9 million viral and over 130 000 plasmid sequences. Identity clustering revealed ∼536 000 candidate viral species, of which 85% are novel relative to existing databases, as well as over 15 000 unique plasmids. Clusters of viral and plasmid proteins were generated de novo, totalling 1 721 182 and 123 813 distinct protein families, respectively. Together, these resources substantially advance previous systematic approaches to characterize anaerobic digestion microbiomes, providing an expanded foundation for investigating the ecological and evolutionary roles of the virome and plasmidome in methane-producing ecosystems. CAnDi is accessible via https://www.candi-db.org/.